2026

• Liloku, P., Alvarez Melo, D., Gisbert, V., Mannaert, S., Mechleb, K., Derclaye, S., Ray, A., Alsteens, D., Volkov, A.N., Sterckx, Y.G.J. & Claeys Bouuaert, C. Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2. Nucleic Acids Res. 54, gkag785 (2026).

• Chen, Y., Bhattacharya, S., Bergmann, L., Correy, G.J., Tan, S.K., Hou, K., Biel, J.T., Lu, L., Bakanas, I., Gestwicki, J.E., Volkov, A.N., Korendovych, I.V., Polizzi, N.F., Fraser, J.S. & DeGrado, W.F. Emergence of specific binding and catalysis from a designed generalist binding protein. Nat.Chem. 18, 1334-44 (2026).

• Priyadarshini, P., Survi, M., El Yazidi Mouloud, W., Bohn, R., Ballet, S., Hunter, N., Volkov, A.N. & Claeys Bouuaert, C. Recruitment of Mre11 to recombination sites during meiosis.  Nat.Commun. 17, 4937 (2026).

• Nembot, M.W., Feller, G., Volkov, A.N., le Paige, U.B., Bouvignies, G. & Damblon, C. Structural and functional insights into a mesophilic cold shock protein CspA with enhanced precision.  J.Magn.Reson. 388, 108077 (2026).

• Pedrelli, G., Chikerema, M., Sokolovskii, A., Lazar, T., Shkumatov, A.V., Volkov, A.N. & Vorobieva, A.A. Negative design enables cell-free expression and folding of designed transmembrane β-barrels.  Proc.Natl.Acad.Sci.U.S.A. 123, e2528772123 (2026).

• Zhang, Z., van der Kant, R., Marković, I., Vizarraga, D., Garcia, T., Maragkou, T., Delgado Blanco, J., Cianferoni, D., Orlando, G., Cia. G., Geukens, N., Carolis, C., Volkov, A.N., Savvides, S.N., Dewilde, M., Schymkowitz, J., Serrano Pubul, L. & Rousseau, F. In silico design of stable single domain antibodies with high affinity. Structure 34, 404-413 (2026).

2025

• Puttemans, J., Brammerloo, Y., Blibek, K., Blavier, J., Ntombela, T., Van Molle, I., Joseph, J., Olivet, J., Saha, D., Degey, M., Hamaidia, M., Jain, P., Piel, G., Zimmermann, P., Kim, D.K., Baiwir, D., Njock, M. S., Dequiedt, F., Salehi-Ashtiani, K., Ballet, S., Volkov, A.N., Twizere, J.C. & Maseko, S.B. Inhibitors of Tax1-PDZ Interactions Block HTLV-1 Viral Transmission by Changing EV Composition. J.Extracell.Vesicles 14, e70137 (2025).

• Lacchini, E., Qu, T., Moses, T., Volkov, A.N. & Goossens, A. Engineering Gypsophila elegans hairy root cultures to produce endosomal escape-enhancing saponins. Plant Biotechnol.J. 23, 3068 (2025).

2024

• Fischer, B., Uchański, T., Sheryazdanova, A. Gonzalez, S., Volkov, A.N., Brosens, E., Zögg, T., Kalichuk, V., Ballet, S., Versées, W., Sablina, A.A., Pardon, E., Wohlkönig, A. & Steyaert, J. Allosteric nanobodies to study the interactions between SOS1 and RAS. Nat.Commun. 15, 6214 (2024).

•  Muwonge, K., Yaman, B., Mészáros, A., Russo, G., Volkov, A.N. & Tompa, P. Improved expression of aggregation-prone Tau proteins using a spidroin-derived solubility tag. Separations 11, 198 (2024).

• Guria, S., Volkov, A.N., Khudaverdyan, R., Van Lommel, R., Pan, R., Daniliuc, C., De Proft, F. & Hennecke, U. Enantioselective, Brønsted acid-catalyzed anti-selective hydroamination of alkenes. J.Am.Chem.Soc. 146, 17180-8 (2024).

• Sülzen, H., Volkov, A.N., Geens, R., Zahedifard, F., Stijlemans, B., Zoltner, M., Magez, S., Sterckx, Y.G.J. &  Zoll, S. Beyond the VSG layer: exploring the role of intrinsic disorder in the invariant surface glycoproteins of African trypanosomes. PLoS Pathog. 20, e1012186 (2024).

• Dhillon, A., Persson, B.D., Volkov, A.N., Sülzen, H., Kádek, A., Pompach, P., Kereïche, S., Lepšik, M., Danskog, K., Uetrecht, C., Arnberg, N. & Zoll, S. Structural insights into the interaction between adenovirus C5 hexon and human lactoferrin. J.Virol. 98, e01576-23 (2024).

• Hadži, S., Živič, Z., Kovačič, M., Zavrtanik, U., Haesaerts, S., Charlier, D., Plavec, J., Volkov, A.N., Lah, J. & Loris, R. Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat.Commun. 15, 3105 (2024).

• Garcia-Rodriguez, G., Girardin, Y., Singh, R.K., Volkov, A.N., Van Dyck, J., Muruganandam, G., Sobott, F., Charlier, D. & Loris, R. Toxin-antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci.Adv. 10, eadj2403 (2024).

• Geens, R., Stanisich, J., Beyens, O., D’Hondt, S., Thiberge, J.M., Ryckebosch, A., De Groot, A., Magez, S., Vertommen, D., Amino, R., De Winter, H., Volkov, A.N., Tompa, P. & Sterckx, Y.G.J. Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain. Prot.Sci. 33, e4852 (2024).

2023

• Caccamo, A., Vega de Luna, F., Wahni, K., Volkov, A.N., Przybyla-Toscano, J., Amelii, A., Kriznik, A., Rouhier, N., Messens, J. & Remacle, C. Ascorbate peroxidase 2 (APX2) of Chlamydomonas binds copper and modulates the copper insertion into plastocyanin. Antioxidants 12, 1946 (2023).

• Maseko, S.B., Brammerloo, Y., Van Molle, I., Sogues, A., Martin, C., Gorgulla, C., Plant, E., Olivet, J., Blavier, J., Ntombela, T., Delvigne, F., Arthanari, H., El Hajj, H., Bazarbachi, A., Van Lint, C., Salehi-Ashtiani, K., Remaut, H., Ballet, S., Volkov, A.N. & Twizere, J.C. Identification of small molecule antivirals against HTLV-1 by targeting the hDLG1-Tax-1 protein-protein interaction. Antivir.Res. 217, 105675 (2023).

• Daccache, D., De Jonge, E., Liloku, P., Mechleb, K., Haddad, M., Corthaut, S., Sterckx, Y.G.J., Volkov, A.N. & Claeys Bouuaert, C. Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev. 37, 535-53 (2023).

•  Prolič-Kalinšek, M.,  Volkov, A.N., Hadži, S.,  Van Dyck, J., Bervoets, I., Charlier, D. & Loris, R. Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7. Acta Cryst. D. 79, 245-58 (2023).

•  Sleutel, M., Pradhan, B., Volkov, A.N. & Remaut, H. Structural analysis and architectural principles of the bacterial amyloid curli. Nat.Commun. 14, 2822 (2023).

•  Haufroid, M., Volkov, A.N. & Wouters, J. Targeting the phosphoserine phosphatase MtSerB2 for tuberculosis drug discovery, an hybrid knowledge based /fragment based approach. Eur.J.Med.Chem. 245, 114935 (2023).

2022

•  Bhattacharya, S., Margheritis, E.G., Takahashi, K., Kulesha, A., D'Souza, A., Kim, I., Tame, J., Yoon, J.H., Volkov, A.N., Makhlynets, O.V. & Korendovych, I.V. NMR-guided directed evolution. Nature 610, 389-93 (2022).

•  Ernst, S., Volkov, A.N., Stark, M., Hölscher, L., Steinert, K., Fetzner, S., Hennecke, U. & Drees, L.S. Azetidomonamide and diazetidomonapyridone metabolites control biofilm formation and pigment synthesis in Pseudomonas aeruginosa. J.Am.Chem.Soc. 144, 7676-85 (2022).

•  Olivet, J., Maseko, S.B., Volkov, A.N., Salehi-Ashtiani, K., Das, K., Calderwood, M.A., Twizere, J.C. & Gorgulla, C. A systematic approach to identify host targets and rapidly deliver broad-spectrum antivirals. Mol.Therapy 30, 1797-800 (2022).

2021

•  Ben Bdira, F., Erkelens, A., Qin, L., Volkov, A.N.,  Andrew, L., Bowring, N., Boyle, A., Ubbink, M., Dove, S. & Dame, R.  Novel anti-repression mechanism of H-NS proteins by a phage protein. Nucleic Acids Res. 49, 10770-84 (2021).

•  Garcia-Rodriguez, G., Girardin, Y., Volkov, A.N., Singh, R.K., Muruganandam, G., Van Dyck, J., Sobott, F., Versées, W., Charlier, D. & Loris, R.  Entropic pressure controls oligomerization of Vibrio cholerae ParD2 antitoxin. Acta Cryst. D 77, 904-20 (2021).

•  Peker, E., Erdogan, A.J., Volkov, A.N. & Riemer, J. Erv1 and cytochrome c mediate rapid electron transfer via a collision-type interaction. J.Mol.Biol. 433, 167045 (2021).

2020

•  Ben Bdira, F., Waudby, C.A., Volkov, A.N., Schröder, S.P., AB, E., Codée, J.D.C., Overkleeft, H.S., Aerts, J.M.F.G., van Ingen, H. & Ubbink. M. Dynamics of ligand binding to a rigid glycosidase. Angew.Chem.Int.Ed. 59, 20508-14 (2020).

•  Tossounian, M.A., Truong, A.C., Buts, L., Wahni, K., Mourenza, A., Leermakers, M., Vertommen, D., Mateos, L.M., Volkov, A.N. & Messens, J. Methionine sulfoxide reductase B from Corynebacterium diphtheriae catalyzes sulfoxide reduction via an intramolecular disulfide cascade. J.Biol.Chem. 295, 3664-77 (2020).

•  Qin, L., Ben Bdira, F.,  Sterckx, Y.G.J., Volkov, A.N., Vreede, J., Giachin, G., van Schaik, P., Ubbink, M. & Dame, R. Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins. Nucleic Acids Res. 48, 2156-72 (2020).

•  Prolič-Kalinšek, M., De Bruyn, P., Juréna, D., Van Melderen, L., Loris, R. & Volkov, A.N. 1H, 13C, and 15N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family. Biomol. NMR Assign. 14, 25-30 (2020).

2019

•  Makhlynets, O., Yoon, J.H., Kulesha, A.V., Lengyel-Zhand, Z., Volkov, A.N., Rempillo, J.J., D’Souza, A., Costeas, C., Chester, C. & Caselle, E.R. Uno Ferro, a de novo designed protein, binds transition metals with high affinity and stabilizes semiquinone radical anion. Chem.Eur.J. 25, 15252-6 (2019).

•  Caselle, E.A., Yoon, J.H., Bhattacharya, S., Rempillo, J.J.L., Lengyel, Z., D'Souza, A., Moroz, Y.S., Tolbert P.L., Volkov, A.N., Forconi, M., Castañeda, C.A., Makhlynets, O.V. & Korendovych, I.V. Kemp Eliminases of the AlleyCat Family Possess High Substrate Promiscuity. Chem.Cat.Chem. 11, 1425-30 (2019).

•  Rice, H.C., de Malmazet, D., Schreurs, A., Frere, S., Van Molle, I., Volkov, A.N., Creemers, E., Vertkin, I., Nys, J., Ranaivoson, F.M., Comoletti, D., Savas, J.N., Remaut, H., Balschun, D., Wierda, K.D., Slutsky, I., Farrow, K., De Strooper, B. & de Wit, J. Secreted Amyloid-? Precursor Protein Functions as a GABABR1a Ligand to Modulate Synaptic Transmission. Science 363, eaao4827 (2019).

2018

•  Nguyen, H.H., Volkov, A.N., Vandenbussche, G., Tompa, P. & Pauwels, K. In vivo biotinylated calpastatin improves the affinity purification of human m-calpain. Prot.Expr.Purif. 145, 77-84 (2018).

•  Kieken, F., Loth, K., van Nuland, N., Tompa, P. & Lenaerts, T. Chemical shift assignment of the partially deuterated Fyn SH2-SH3 domain. Biomol.NMR Assign. 12, 117-22 (2018).

2017

•  Contreras-Martos, S., Piai, A., Kosol, S., Varadi, M., Bekesi, A., Lebrun, P., Volkov, A.N., Gevaert, K., Pierattelli, R., Felli, I. & Tompa, P. Linking functions: an additional role for an intrinsically disordered linker domain in the transcriptional coactivator CBP. Sci.Rep. 7, 4676 (2017).

•  Boeynaems, S., Bogaert, E., Kovacs, D., Konijnenberg, A., Timmerman, E., Volkov, A., Guharoy, M., De Decker, M., Jaspers, T., Ryan, V.H., Janke, A.M., Baatsen, P., Vercruysse, T., Kolaitis, R.M., Daelemans, D., Taylor, J.P., Kedersha, N., Anderson, P., Impens, F., Sobott, F., Schymkowitz, J., Rousseau, F., Fawzi, N.L., Robberecht, W., Van Damme, P., Tompa, P. & Van Den Bosch, L. Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics. Mol.Cell 65, 1044-55 (2017).

2016

•  Antonik, P.M., Volkov, A.N., Broder, U.N., Lo Re, D., van Nuland, N.A.J. & Crowley, P.B. Anomer-specific recognition and dynamics in a fucose-binding lectin. Biochemistry 55, 1195-203 (2016).

• Huculeci, R., Cilia, E., Lyczek, A., Buts, L., Houben, K., Seeliger M.A., van Nuland, N.A.J. & Lenaerts, T. Dynamically coupled residues within the SH2 domain of FYN are key to unlocking its activity. Structure 24, 1947-59 (2016).

• Hubin, E., Cioffi, F., Rozenski, J., van Nuland, N.A.J. & Broersen, K. (2016) Characterization of insulin-degrading enzyme-mediated cleavage of Aß in distinct aggregation states. Biochim.Biophys, Acta 1860, 1281-90 (2016).

2015

• Volkov, A.N. Structure and function of transient encounters of redox proteins. Acc.Chem.Res. 48, 3036-43 (2015).

• Van de Water, K., Sterckx, Y.G.J. & Volkov, A.N. The low-affinity complex of cytochrome c and its peroxidase. Nat.Commun. 6, 7073 (2015).

• Moroz, Y.S., Dunston, T.T., Makhlynets, O.V., Moroz, O.V., Wu, Y., Yoon, J.H., Olsen, A.B., McLaughlin, J.M., Mack, K.L., Gosavi, P.M., van Nuland, N.A.J. & Korendovych, I.V. (2015) New tricks for old proteins: single mutations in a nonenzymatic protein give rise to various enzymatic activities. J.Am.Chem.Soc. 137, 14905-11 (2015).

• Huculeci, R., Garcia-Pino, A., Buts, L., Lenaerts, T. & van Nuland, N.A.J. Structural insights into the intertwined dimer of fyn SH2. Protein Sci. 24, 1964-78 (2015).

• Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.G.J., Respondek, M., Engelberg-Kulka, H., Loris, R., Zangger, K. & van Nuland, N.A.J. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding. Nucleic Acids Res. 43, 1241-58 (2015).

before 2015

• Van de Water, K., van Nuland, N.A.J & Volkov, A.N. Transient protein encounters characterized by paramagnetic NMR. Chem.Sci. 5, 4227-36 (2014).

• Sterckx, Y.G.J. & Volkov, A.N. Cofactor-dependent structural and binding properties of yeast cytochrome c peroxidase. Biochemistry 53, 4526-36 (2014).

• Sterckx, Y.G.J., Volkov, A.N., Vranken, W.F., Kragelj, J., Ringkjøbing-Jensen, M., Buts, L., Garcia-Pino, A., Jové, T., Van Melderen, L., Blackledge, M., van Nuland, N.A.J. & Loris, R. SAXS and NMR-derived conformational ensemble of the highly flexible antitoxin PaaA2. Structure 22, 854-65 (2014).

• Vanwetswinkel, S., Volkov, A.N., Sterckx, Y.G.J., Garcia-Pino, A., Buts, L., Vranken, W.F., Bouckaert, J., Roy, R., Wyns, L. & van Nuland, N.A.J. Study of the structural and dynamic effects in the FimH adhesin upon α-D-heptyl mannose binding. J.Med.Chem. 57, 1416-27 (2014).

• Zorzini, V., Buts, L., Sleutel, M., Garcia-Pino, A., Talavera, A., Haesaerts, S., De Greve, H., Cheung, A, van Nuland, N.A.J. & Loris, R. Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics. Nucleic Acids Res. 42, 6709-25 (2014).

• Ceregido, M.A., Spínola Amilibia, M., Buts, L., Rivera-Torres, J., Garcia-Pino, A., Bravo, J. & van Nuland, N.A.J. The structure of TAX1BP1 UBZ1+2 provides insight into target specificity and adaptability. J.Mol.Biol. 426, 674-90 (2014).

• Rubio, L., Huculeci, R., Buts, L., Vanwetswinkel, S., Lenaerts, T. & van Nuland, N.A.J. 1H, 13C, and 15N backbone and side-chain chemical shift assignments of the free and bound forms of the human PTPN11 second SH2 domain. Biomol.NMR.Assign. 8, 297-301 (2014).

• De Gieter, S., Loris, R., van Nuland, N.A.J. & Garcia-Pino, A. 1H, 13C, and 15N backbone and sidechain chemical shift assignments of the toxin Doc in the unbound state. Biomol.NMR.Assign. 8, 145-8 (2014).

• Vanwetswinkel, S., van Nuland, N.A.J. & Volkov, A.N. Paramagnetic properties of the low- and high-spin states of yeast cytochrome c peroxidase. J.Biomol.NMR 57, 21-6 (2013).

• Volkov, A.N. & van Nuland, N.A.J. Solution NMR study of the yeast cytochrome c peroxidase - cytochrome c interaction. J.Biomol.NMR 56, 255-63 (2013).

• Volkov, A.N., Wohlkonig, A., Soror, S.H. & van Nuland, N.A.J. Expression, purification, characterization, and solution nuclear magnetic resonance study of highly deuterated yeast cytochrome c peroxidase with enhanced solubility. Biochemistry 52, 2165-75 (2013).

• Castro-Roa, D., Garcia-Pino, A., De Gieter, S., van Nuland, N.A.J., Loris, R. & Zenkin, N. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. Nat.Chem.Biol. 9, 811-7 (2013).

• Ortega Roldan, J.L., Casares, S., Ringkjøbing Jensen, M., Cárdenes, N., Bravo, J., Blackledge, M., Azuaga, A.I. & van Nuland, N.A.J. Distinct ubiquitin binding modes exhibited by SH3 domains: molecular determinants and functional implications. PLoS One 8, e73018 (2013).

• Ceregido, M.A., Garcia-Pino, A., Ortega-Roldan, J.L., Casares, S., López Mayorga, O., Bravo, J., van Nuland, N.A.J. & Azuaga, A.I. Multimeric and differential binding of CIN85/CD2AP with two atypical proline-rich sequences from CD2 and Cbl-b. FEBS J. 280, 3399-415 (2013).

• Volkov, A.N. & van Nuland, N.A.J. Electron transfer interactome of cytochrome c. PLoS Comput.Biol. 8, e1002807 (2012).

• Volkov, A.N., Vanwetswinkel, S., Van de Water, K. & van Nuland, N.A.J. Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy. J.Biomol.NMR 52, 245-56 (2012).

• Shah, D.M., AB, E., Diercks, T., Hass, M.A.S., van Nuland, N.A.J. & Siegal, G.D. Rapid protein-ligand co-structures from sparse NOE data. J.Med.Chem. 55, 10786-90 (2012).

• Van Laer, K., Buts, L., Foloppe, N., Vertommen, D., Van Belle, K., Wahni, K., Roos, G., Nilsson, L., Mateos, L.M., Rawat, M., van Nuland, N.A.J. & Messens, J. Mycoredoxin-1 is one of the missing links in the oxidative stress defense mechanism of Mycobacteria. Mol.Microbiol. 86, 787-804 (2012).

• Abskharon, R.N., Ramboarina, S., El Hassan, H., Gad, W., Apostol, M.I., Giachin, G., Legname, G., Steyaert, J., Messens, J., Soror, S.H. & Wohlkonig, A. A novel expression system for production of soluble prion proteins in E. coli. Microb.Cell Fact. 11, 6 (2012).

• Volkov, A.N., Nicholls, P. & Worrall, J.A.R. The complex of cytochrome c and cytochrome c peroxidase: the end of the road? Biochim.Biophys.Acta 1807, 1482-503 (2011).

• Van de Water, K., Soror, S.H., Wohlkonig, A., van Nuland, N.A.J. & Volkov, A.N. Crystallization and preliminary X-ray diffraction analysis of kanamycin-binding beta-lactamase in complex with its ligand. Acta Cryst.F. 67, 703-6 (2011).

• Jensen, M.R., Ortega-Roldan, J.L., Salmon, L., van Nuland, N.A.J. & Blackledge, M. Characterizing weak protein-protein complexes by NMR residual dipolar couplings. Eur.Biophys.J. 40, 1371-81 (2011).

• Ortega-Roldan, J.L., Blackledge, M., van Nuland, N.A.J. & Azuaga, A.I. Solution structure, dynamics and thermodynamics of the three SH3 domains of CD2AP. J.Biomol.NMR 50, 103-17 (2011).

• Huculeci, R., Buts, L., Lenaerts, T. & van Nuland N.A.J. 1H, 13C and 15N backbone and side-chain chemical shift assignment of the Fyn SH2 domain and its complex with a phosphotyrosine peptide. Biomol.NMR.Assign. 5, 181-4 (2011).

• Zorzini, V., Haesaerts, S., Cheung, A., Loris, R. & van Nuland, N.A.J. 1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase. Biomol.NMR.Assign. 5, 157-60 (2011).

• Volkov, A.N., Ubbink, M. & van Nuland, N.A.J. Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy. J.Biomol.NMR 48, 225-36 (2010).

• Garcia-Pino, A., Balasubramanian, S., Wyns, L., Gazit, E., De Greve, H., Magnuson, R.D., Charlier, D., van Nuland, N.A.J. & Loris R. Allostery and intrinsic disorder mediate transcription regulation by conditional cooperativity. Cell 142, 101-11 (2010).