61. Liloku, P., Alvarez Melo, D., Gisbert, V., Mannaert, S., Mechleb, K., Derclaye, S., Ray, A., Alsteens, D., Volkov, A.N., Sterckx, Y.G.J. & Claeys Bouuaert, C. Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2. Nucleic Acids Res. 54, gkag785 (2026).

60. Chen, Y., Bhattacharya, S., Bergmann, L., Correy, G.J., Tan, S.K., Hou, K., Biel, J.T., Lu, L., Bakanas, I., Gestwicki, J.E., Volkov, A.N., Korendovych, I.V., Polizzi, N.F., Fraser, J.S. & DeGrado, W.F. Emergence of specific binding and catalysis from a designed generalist binding protein. Nat.Chem. 18, 1334-44 (2026).

59. Priyadarshini, P., Survi, M., El Yazidi Mouloud, W., Bohn, R., Ballet, S., Hunter, N., Volkov, A.N. & Claeys Bouuaert, C. Recruitment of Mre11 to recombination sites during meiosis.  Nat.Commun. 17, 4937 (2026).

58. Nembot, M.W., Feller, G., Volkov, A.N., le Paige, U.B., Bouvignies, G. & Damblon, C. Structural and functional insights into a mesophilic cold shock protein CspA with enhanced precision.  J.Magn.Reson. 388, 108077 (2026).

57. Pedrelli, G., Chikerema, M., Sokolovskii, A., Lazar, T., Shkumatov, A.V., Volkov, A.N. & Vorobieva, A.A. Negative design enables cell-free expression and folding of designed transmembrane β-barrels.  Proc.Natl.Acad.Sci.U.S.A. 123, e2528772123 (2026).

56. Zhang, Z., van der Kant, R., Marković, I., Vizarraga, D., Garcia, T., Maragkou, T., Delgado Blanco, J., Cianferoni, D., Orlando, G., Cia. G., Geukens, N., Carolis, C., Volkov, A.N., Savvides, S.N., Dewilde, M., Schymkowitz, J., Serrano Pubul, L. & Rousseau, F. In silico design of stable single domain antibodies with high affinity. Structure 34, 404-413 (2026).

55. Puttemans, J., Brammerloo, Y., Blibek, K., Blavier, J., Ntombela, T., Van Molle, I., Joseph, J., Olivet, J., Saha, D., Degey, M., Hamaidia, M., Jain, P., Piel, G., Zimmermann, P., Kim, D.K., Baiwir, D., Njock, M. S., Dequiedt, F., Salehi-Ashtiani, K., Ballet, S., Volkov, A.N., Twizere, J.C. & Maseko, S.B. Inhibitors of Tax1-PDZ Interactions Block HTLV-1 Viral Transmission by Changing EV Composition. J.Extracell.Vesicles 14, e70137 (2025).

54. Lacchini, E., Qu, T., Moses, T., Volkov, A.N. & Goossens, A. Engineering Gypsophila elegans hairy root cultures to produce endosomal escape-enhancing saponins. Plant Biotechnol.J. 23, 3068 (2025).

53. Fischer, B., Uchański, T., Sheryazdanova, A. Gonzalez, S., Volkov, A.N., Brosens, E., Zögg, T., Kalichuk, V., Ballet, S., Versées, W., Sablina, A.A., Pardon, E., Wohlkönig, A. & Steyaert, J. Allosteric nanobodies to study the interactions between SOS1 and RAS. Nat.Commun. 15, 6214 (2024).

52. Muwonge, K., Yaman, B., Mészáros, A., Russo, G., Volkov, A.N. & Tompa, P. Improved expression of aggregation-prone Tau proteins using a spidroin-derived solubility tag. Separations 11, 198 (2024). BMRB(52503)

51. Guria, S., Volkov, A.N., Khudaverdyan, R., Van Lommel, R., Pan, R., Daniliuc, C., De Proft, F. & Hennecke, U. Enantioselective, Brønsted acid-catalyzed anti-selective hydroamination of alkenes. J.Am.Chem.Soc. 146, 17180-8 (2024).

50. Sülzen, H., Volkov, A.N., Geens, R., Zahedifard, F., Stijlemans, B., Zoltner, M., Magez, S., Sterckx, Y.G.J. &  Zoll, S. Beyond the VSG layer: exploring the role of intrinsic disorder in the invariant surface glycoproteins of African trypanosomes. PLoS Pathog. 20, e1012186 (2024).

49. Dhillon, A., Persson, B.D., Volkov, A.N., Sülzen, H., Kádek, A., Pompach, P., Kereïche, S., Lepšik, M., Danskog, K., Uetrecht, C., Arnberg, N. & Zoll, S. Structural insights into the interaction between adenovirus C5 hexon and human lactoferrin. J.Virol. 98, e01576-23 (2024).

48. Hadži, S., Živič, Z., Kovačič, M., Zavrtanik, U., Haesaerts, S., Charlier, D., Plavec, J., Volkov, A.N., Lah, J. & Loris, R. Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat.Commun. 15, 3105 (2024). PDB(8A0W) BMRB(51448)

47. Garcia-Rodriguez, G., Girardin, Y., Singh, R.K., Volkov, A.N., Van Dyck, J., Muruganandam, G., Sobott, F., Charlier, D. & Loris, R. Toxin-antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci.Adv. 10, eadj2403 (2024). PDB(7R5A)

46. Geens, R., Stanisich, J., Beyens, O., D’Hondt, S., Thiberge, J.M., Ryckebosch, A., De Groot, A., Magez, S., Vertommen, D., Amino, R., De Winter, H., Volkov, A.N., Tompa, P. & Sterckx, Y.G.J. Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain. Prot.Sci. 33, e4852 (2024).

45. Caccamo, A., Vega de Luna, F., Wahni, K., Volkov, A.N., Przybyla-Toscano, J., Amelii, A., Kriznik, A., Rouhier, N., Messens, J. & Remacle, C. Ascorbate peroxidase 2 (APX2) of Chlamydomonas binds copper and modulates the copper insertion into plastocyanin. Antioxidants 12, 1946 (2023).

44. Maseko, S.B., Brammerloo, Y., Van Molle, I., Sogues, A., Martin, C., Gorgulla, C., Plant, E., Olivet, J., Blavier, J., Ntombela, T., Delvigne, F., Arthanari, H., El Hajj, H., Bazarbachi, A., Van Lint, C., Salehi-Ashtiani, K., Remaut, H., Ballet, S., Volkov, A.N.* & Twizere, J.C.* Identification of small molecule antivirals against HTLV-1 by targeting the hDLG1-Tax-1 protein-protein interaction. Antivir.Res. 217, 105675 (2023). PDB(8CN1) PDB(8CN3) *Corresponding authors

43. Daccache, D., De Jonge, E., Liloku, P., Mechleb, K., Haddad, M., Corthaut, S., Sterckx, Y.G.J., Volkov, A.N.* & Claeys Bouuaert, C.* Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev. 37, 535-53 (2023). BMRB(26335) *Corresponding authors

42.  Prolič-Kalinšek, M.,  Volkov, A.N., Hadži, S.,  Van Dyck, J., Bervoets, I., Charlier, D. & Loris, R. Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7. Acta Cryst. D. 79, 245-58 (2023).

41.  Sleutel, M., Pradhan, B., Volkov, A.N. & Remaut, H. Structural analysis and architectural principles of the bacterial amyloid curli. Nat.Commun. 14, 2822 (2023).

40.  Haufroid, M., Volkov, A.N. & Wouters, J. Targeting the phosphoserine phosphatase MtSerB2 for tuberculosis drug discovery, an hybrid knowledge based /fragment based approach. Eur.J.Med.Chem. 245, 114935 (2023).

39.  Bhattacharya, S., Margheritis, E.G., Takahashi, K., Kulesha, A., D'Souza, A., Kim, I., Tame, J., Yoon, J.H., Volkov, A.N.*, Makhlynets, O.V.* & Korendovych, I.V.* NMR-guided directed evolution. Nature 610, 389-93 (2022). *Corresponding authors

38.  Ernst, S., Volkov, A.N., Stark, M., Hölscher, L., Steinert, K., Fetzner, S., Hennecke, U. & Drees, L.S. Azetidomonamide and diazetidomonapyridone metabolites control biofilm formation and pigment synthesis in Pseudomonas aeruginosa. J.Am.Chem.Soc. 144, 7676-85 (2022).

37.  Olivet, J., Maseko, S.B., Volkov, A.N., Salehi-Ashtiani, K., Das, K., Calderwood, M.A., Twizere, J.C. & Gorgulla, C. A systematic approach to identify host targets and rapidly deliver broad-spectrum antivirals. Mol.Therapy 30, 1797-800 (2022).

36.  Ben Bdira, F., Erkelens, A., Qin, L., Volkov, A.N.,  Andrew, L., Bowring, N., Boyle, A., Ubbink, M., Dove, S. & Dame, R.  Novel anti-repression mechanism of H-NS proteins by a phage protein. Nucleic Acids Res. 49, 10770-84 (2021). PDB(6YSE) BMRB(28112)

35.  Garcia-Rodriguez, G., Girardin, Y., Volkov, A.N., Singh, R.K., Muruganandam, G., Van Dyck, J., Sobott, F., Versées, W., Charlier, D. & Loris, R.  Entropic pressure controls oligomerization of Vibrio cholerae ParD2 antitoxin. Acta Cryst. D 77, 904-20 (2021).

34.  Peker, E., Erdogan, A.J., Volkov, A.N.* & Riemer, J.* Erv1 and cytochrome c mediate rapid electron transfer via a collision-type interaction. J.Mol.Biol. 433, 167045 (2021). BMRB(50649) *Corresponding authors

33.  Ben Bdira, F., Waudby, C.A., Volkov, A.N., Schröder, S.P., AB, E., Codée, J.D.C., Overkleeft, H.S., Aerts, J.M.F.G., van Ingen, H. & Ubbink. M. Dynamics of ligand binding to a rigid glycosidase. Angew.Chem.Int.Ed. 59, 20508-14 (2020).

32.  Tossounian, M.A., Truong, A.C., Buts, L., Wahni, K., Mourenza, A., Leermakers, M., Vertommen, D., Mateos, L.M., Volkov, A.N. & Messens, J. Methionine sulfoxide reductase B from Corynebacterium diphtheriae catalyzes sulfoxide reduction via an intramolecular disulfide cascade. J.Biol.Chem. 295, 3664-77 (2020). PDB(6TR8) BMRB(28052)

31.  Qin, L., Ben Bdira, F.,  Sterckx, Y.G.J., Volkov, A.N., Vreede, J., Giachin, G., van Schaik, P., Ubbink, M. & Dame, R. Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins. Nucleic Acids Res. 48, 2156-72 (2020).

30.  Prolič-Kalinšek, M., De Bruyn, P., Juréna, D., Van Melderen, L., Loris, R. & Volkov, A.N. 1H, 13C, and 15N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family. Biomol. NMR Assign. 14, 25-30 (2020). BMRB(27917)

29.  Makhlynets, O., Yoon, J.H., Kulesha, A.V., Lengyel-Zhand, Z., Volkov, A.N., Rempillo, J.J., D’Souza, A., Costeas, C., Chester, C. & Caselle, E.R. Uno Ferro, a de novo designed protein, binds transition metals with high affinity and stabilizes semiquinone radical anion. Chem.Eur.J. 25, 15252-6 (2019). BMRB(27481)

28.  Caselle, E.A., Yoon, J.H., Bhattacharya, S., Rempillo, J.J.L., Lengyel, Z., D'Souza, A., Moroz, Y.S., Tolbert P.L., Volkov, A.N., Forconi, M., Castañeda, C.A., Makhlynets, O.V. & Korendovych, I.V. Kemp Eliminases of the AlleyCat Family Possess High Substrate Promiscuity. Chem.Cat.Chem. 11, 1425-30 (2019).

Front cover

27.  Rice, H.C., de Malmazet, D., Schreurs, A., Frere, S., Van Molle, I., Volkov, A.N., Creemers, E., Vertkin, I., Nys, J., Ranaivoson, F.M., Comoletti, D., Savas, J.N., Remaut, H., Balschun, D., Wierda, K.D., Slutsky, I., Farrow, K., De Strooper, B. & de Wit, J. Secreted Amyloid-? Precursor Protein Functions as a GABABR1a Ligand to Modulate Synaptic Transmission. Science 363, eaao4827 (2019). PDB(6HKC) BMRB(27581)
     Science Perspective (Science 363, 123-124)
     Neuron Spotlight (Neuron 101, 557-559)
     PNAS Inner Workings (Proc.Natl.Acad.Sci.USA 116, 11082-11084)

26.  Nguyen, H.H., Volkov, A.N., Vandenbussche, G., Tompa, P. & Pauwels, K. In vivo biotinylated calpastatin improves the affinity purification of human m-calpain. Prot.Expr.Purif. 145, 77-84 (2018).

25.  Contreras-Martos, S., Piai, A., Kosol, S., Varadi, M., Bekesi, A., Lebrun, P., Volkov, A.N., Gevaert, K., Pierattelli, R., Felli, I. & Tompa, P. Linking functions: an additional role for an intrinsically disordered linker domain in the transcriptional coactivator CBP. Sci.Rep. 7, 4676 (2017). BMRB(26841)

24.  Boeynaems, S., Bogaert, E., Kovacs, D., Konijnenberg, A., Timmerman, E., Volkov, A., Guharoy, M., De Decker, M., Jaspers, T., Ryan, V.H., Janke, A.M., Baatsen, P., Vercruysse, T., Kolaitis, R.M., Daelemans, D., Taylor, J.P., Kedersha, N., Anderson, P., Impens, F., Sobott, F., Schymkowitz, J., Rousseau, F., Fawzi, N.L., Robberecht, W., Van Damme, P., Tompa, P. & Van Den Bosch, L. Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics. Mol.Cell 65, 1044-55 (2017).

Front cover

23.  Antonik, P.M.*, Volkov, A.N.*, Broder, U.N., Lo Re, D., van Nuland, N.A.J. & Crowley, P.B. Anomer-specific recognition and dynamics in a fucose-binding lectin. Biochemistry 55, 1195-203 (2016). *contributed equally BMRB(25950) BMRB(25951) BMRB(25952)  

22. Volkov, A.N.* Structure and function of transient encounters of redox proteins. Acc.Chem.Res. 48, 3036-43 (2015). *Corresponding author

21. Van de Water, K., Sterckx, Y.G.J. & Volkov, A.N.* The low-affinity complex of cytochrome c and its peroxidase. Nat.Commun. 6, 7073 (2015). *Corresponding author

20. Van de Water, K., van Nuland, N.A.J & Volkov, A.N.* Transient protein encounters characterized by paramagnetic NMR. Chem.Sci. 5, 4227-36 (2014). *Corresponding author

19. Sterckx, Y.G.J. & Volkov, A.N.* Cofactor-dependent structural and binding properties of yeast cytochrome c peroxidase. Biochemistry 53, 4526-36 (2014). *Corresponding author

18. Sterckx, Y.G.J., Volkov, A.N., Vranken, W.F., Kragelj, J., Ringkjøbing-Jensen, M., Buts, L., Garcia-Pino, A., Jové, T., Van Melderen, L., Blackledge, M., van Nuland, N.A.J. & Loris, R. SAXS and NMR-derived conformational ensemble of the highly flexible antitoxin PaaA2. Structure 22, 854-65 (2014). PDB(3ZBE) BMRB(18841)

17. Vanwetswinkel, S., Volkov, A.N.*, Sterckx, Y.G.J., Garcia-Pino, A., Buts, L., Vranken, W.F., Bouckaert, J., Roy, R., Wyns, L. & van Nuland, N.A.J.* Study of the structural and dynamic effects in the FimH adhesin upon α-D-heptyl mannose binding. J.Med.Chem. 57, 1416-27 (2014). PDB(3ZPD) PDB(4LOV) BMRB(19066) BMRB(19254) BMRB(19255) BMRB(19256) *Corresponding authors

16. Vanwetswinkel, S., van Nuland, N.A.J. & Volkov, A.N.* Paramagnetic properties of the low- and high-spin states of yeast cytochrome c peroxidase. J.Biomol.NMR 57, 21-6 (2013). BMRB(19075) BMRB(19076) *Corresponding author

15. Volkov, A.N.* & van Nuland, N.A.J. Solution NMR study of the yeast cytochrome c peroxidase - cytochrome c interaction. J.Biomol.NMR 56, 255-63 (2013). *Corresponding author

14. Volkov, A.N.*, Wohlkonig, A., Soror, S.H. & van Nuland, N.A.J. Expression, purification, characterization, and solution nuclear magnetic resonance study of highly deuterated yeast cytochrome c peroxidase with enhanced solubility. Biochemistry 52, 2165-75 (2013). PDB(4JB4) BMRB(19004) BMRB(19005) *Corresponding author

13. Volkov, A.N.* & van Nuland, N.A.J. Electron transfer interactome of cytochrome c. PLoS Comput.Biol. 8, e1002807 (2012). *Corresponding author

  12. Volkov, A.N., Vanwetswinkel, S., Van de Water, K. & van Nuland, N.A.J. Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy. J.Biomol.NMR 52, 245-56 (2012). BMRB(17845) BMRB(17846) BMRB(17847) BMRB(17848)

  Front cover

11. Volkov, A.N.*, Nicholls, P. & Worrall, J.A.R. The complex of cytochrome c and cytochrome c peroxidase: the end of the road? Biochim.Biophys.Acta 1807, 1482-503 (2011). *Corresponding author

10. Van de Water, K., Soror, S.H., Wohlkonig, A., van Nuland, N.A.J. & Volkov, A.N. Crystallization and preliminary X-ray diffraction analysis of kanamycin-binding beta-lactamase in complex with its ligand. Acta Cryst.F. 67, 703-6 (2011).

9. Volkov, A.N., Barrios, H., Mathonet, M., Evrard, C., Ubbink, M., Declercq, J.P., Soumillion, P. & Fastrez, J. Engineering an allosteric binding site for aminoglycosides into the TEM-1 beta-lactamase. Chem.Bio.Chem. 12, 904-13 (2011). PDB(2V1Z) PDB(2V20) BMRB(15917)

8. Volkov, A.N.*, Ubbink, M. & van Nuland, N.A.J. Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy. J.Biomol.NMR 48, 225-36 (2010). *Corresponding author

7. Volkov, A.N., Bashir, Q., Worrall, J.A.R., Ullmann, G.M. & Ubbink, M. Shifting the equilibrium between the encounter state and the specific form of a protein complex by interfacial point mutations. J.Am.Chem.Soc. 132, 11487-95 (2010). PDB(2JTI)

6. Bashir, Q., Volkov, A.N., Ullmann, G.M. & Ubbink, M. Visualization of the encounter ensemble of the transient electron transfer complex of cytochrome c and cytochrome c peroxidase. J.Am.Chem.Soc. 132, 241-7 (2010).

5. Yu, D., Volkov, A.N. & Tang, C. Characterizing dynamic protein-protein interactions using differentially scaled paramagnetic relaxation enhancement. J.Am.Chem.Soc. 131, 17291-7 (2009).

4. Volkov, A.N., Bashir, Q., Worrall, J.A.R. & Ubbink, M. Binding hot spot in the weak protein complex of physiological redox partners yeast cytochrome c and cytochrome c peroxidase. J.Mol.Biol. 385, 1003-13 (2009).

  3. Volkov, A.N., Worrall, J.A.R., Holtzmann, E. & Ubbink, M. Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR. Proc.Natl.Acad.Sci.U.S.A. 103, 18945-50 (2006). PDB(2GB8)

  Front cover

2. Volkov, A.N., Ferrari, D., Worrall, J.A.R., Bonvin, A.M.J.J. & Ubbink, M. The orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by NMR and docking using HADDOCK. Prot.Sci. 14, 799-811 (2005).

1. Zaitsev, V.N., Volkov, A.N., Zub, V.Ya. & Zaitseva, G.N. A study of the interaction of diethylenetriamine complexes of copper fixed on silica with reagents of different nature by the ESR method. Ukrainskij Khimicheskij Zhurnal (Russian Edition) 64, 12-7 (1998).